ABSTRACT
Objective To construct and rescue recombinant influenza virus strains expressing hu-man metapneumovirus ( hMPV) epitopes. -ethods B cell, CTL and Th epitopes predicted by bioinformat-ics software were coupled together in different combinations. These different array genes were inserted into the NS1 gene of influenza virus strain A/PR/8/34 ( PR8 ) , respectively. Recombinant PR8 influenza virus vectors expressing different hMPV antigenic epitopes were rescued by reverse genetics using eight-plasmid system. Sequencing analysis was conducted to verify whether the rescued viruses carried the chimeric hMPV epitopes. Hemagglutination ( HA) titers, half tissue culture infection dose ( TCID50 ) and growth curves were detected. Results Interval sequences GPGPG and KK were introduced into hMPV epitope combinations to construct multi-epitope antigens (MEA). These MEA were inserted into the PR8 NS gene, respectively. Using 8 plasmid system, three recombinant influenza virus strains were rescued successfully. After cultured for three passages in Madin-Darby canine kidney ( MDCK) cells and one in eggs, these three recombinant strains could proliferate steadily. Whole genome sequencing verified that the three recombinant strains car-ried the chimeric MEA sequences, named as rFLU/hMPV/B, rFLU/hMPV/CTL-Th and rFLU/hMPV/B-Th. HA titers of the recombinant strains were 128, 128 and 256 using turkey erythrocyte, respectively. Their TCID50 were 107. 0/ml, 106. 8/ml and 107. 0/ml, respectively. Growth curve tests also verified that the recombinant strains could proliferate steadily in MDCK cells. Conclusions Three recombinant influenza vi-rus vector strains carrying the B cell, CTL and Th epitopes of hMPV were rescued successfully. This study lays the foundation for further evaluation of the immune effects of these recombinant viruses and their poten-tial application value in vaccine development.
ABSTRACT
The present study aimed to clone and express the EmLDH gene of Echinococcus multilocularis in Qinghai Province,identifying immunogenicity of EmLDH recombinant protein and evaluating its immune diagnostic value preliminarily.EmLDH genes were cloned by RT-PCR technology and linked into pET15b vector.Recombinant expression pET15b-EmLDH vectors were constructed and transformed into E.coli Rosetta (DE3) competent cells.Recombinant proteins were induced and expressed.Expression forms of recombinant proteins were detected by SDS-PAGE.Recombinant proteins were purified by affinity chromatography of Ni-IDA resin.Immunogenicity of recombinant proteins was identified by Western blotting.Serum samples from patients with alveolar echinococcosis (57 cases),cystic echinococcosis (33 cases),and healthy persons (50 cases) were examined by ELISA,which evaluated preliminarily immune diagnosis effect of EmLDH recombinant proteins.Results showed that EmLDH gene was cloned successfully and the recombinant proteins were expressed and purified.Results of Western blotting showed EmLDH recombinant proteins were recognised by serum samples from patients with alveolar echinococcosis and cystic echinococcosis,but not by serum samples from healthy persons.Results of ELISA showed that diagnostic sensitivities of EmLDH recombinant protein reacted with serum samples from patients with alveolar echinococcosis and cystic echinococcosis were 84.21％ and 84.85 ％ respectively.EmLDH recombinant proteins of Echinococcus multilocularis have high immunogenicity and good immune diagnostic value for echinococcosis.
ABSTRACT
Objective To study the Hengkangzhengqing combined application of psychological intervention in the bowel preparation before colonoscopy. Methods 400 cases of colonoscopy patients from January 2016 to December 2016 were selected and randomly divided into the control group and the experimental group, each with 200 cases. The control group were treated with Hengkangzhengqing, experimental group on the basis of psychological intervention on the mental status of patients and patients, strengthen communication and exchanges, increase the treatment compliance of patients. Comparative analysis of the two groups of patients related clinical indicators. Results In the control group, 100 cases of intestinal cleaning efficacy of 0 scores, and 80 cases of 1 scores. In the experimental group, 80 cases had clean bowel effect, and 70 cases were 1 scores. The intestinal cleaning effect of the experimental group was 90.0%, the percentage of the control group 0~1 scores was 75.0%, and the intestinal cleaning effect of the experimental group was significantly better than that of the control group, with statistical significance (P<0.05) (0-1 scores). The adverse reaction rate in the experimental group was 8.0%, significantly lower than that in the control group (22.0%), with statistical significance (P<0.05). In the control group, 80 cases received excellent bowel preparation, and 70 cases were good. In the experimental group, 110 patients received excellent intestinal preparation and 82 patients were good, the excellent rate of the 2 groups was statistically significant (P<0.05). Conclusion Hengkangzhengqing combined with psychological intervention in a safe colonoscopy application in bowel preparation, can significantly improve the cleaning effect and acceptability, has clinical significance.
ABSTRACT
Objective To study the Hengkangzhengqing combined application of psychological intervention in the bowel preparation before colonoscopy. Methods 400 cases of colonoscopy patients from January 2016 to December 2016 were selected and randomly divided into the control group and the experimental group, each with 200 cases. The control group were treated with Hengkangzhengqing, experimental group on the basis of psychological intervention on the mental status of patients and patients, strengthen communication and exchanges, increase the treatment compliance of patients. Comparative analysis of the two groups of patients related clinical indicators. Results In the control group, 100 cases of intestinal cleaning efficacy of 0 scores, and 80 cases of 1 scores. In the experimental group, 80 cases had clean bowel effect, and 70 cases were 1 scores. The intestinal cleaning effect of the experimental group was 90.0%, the percentage of the control group 0~1 scores was 75.0%, and the intestinal cleaning effect of the experimental group was significantly better than that of the control group, with statistical significance (P<0.05) (0-1 scores). The adverse reaction rate in the experimental group was 8.0%, significantly lower than that in the control group (22.0%), with statistical significance (P<0.05). In the control group, 80 cases received excellent bowel preparation, and 70 cases were good. In the experimental group, 110 patients received excellent intestinal preparation and 82 patients were good, the excellent rate of the 2 groups was statistically significant (P<0.05). Conclusion Hengkangzhengqing combined with psychological intervention in a safe colonoscopy application in bowel preparation, can significantly improve the cleaning effect and acceptability, has clinical significance.